The Enzymatic Preparation of Human Milk Fat Substitute Intermediate Rich in Palmitic Acid at <i>sn</i>-2 Position and Low-Unsaturated Fatty Acids at <i>sn</i>-1(3) Positions from Palm Oil Substrate

نویسندگان

چکیده

The lipid products that consist of structured lipids rich in palmitic acid (16:0) at the sn-2 position triacylglycerol (TAG) and low-unsaturated fatty acids (FAs) (LUFAs), such as oleic acid; 18:1 linoleic 18:2 sn-1(3) positions, are useful intermediates for manufacturing human milk fat substitute (HMFS), which contains functional components. In this study, HMFS intermediate (HMFS-IM) was enzymatically prepared from palm oil without using other sources. First, amount 16:0 TAG substrate enhanced 18.9% to more 34.5% via a random esterification reaction non-stereospecific lipase, Novozym® 435, produce random-palm substrate. Consequently, 2-monoacylglycerol (2-MAG) over 88%, together with FA ethyl ester substrates LUFAs, 18:1-Et 18:2-Et above 93.5% through ethanolysis same lipase by purification urea complexation, respectively. As preferred modified method, continuous use these reactions were achieved while reducing usage enzyme half. Finally, an HMFS-IM than 60% LUFA positions oil-based products, including random-palm, palm-Et, 2-MAG, interesterification 1,3-stereospecific Lipozyme® RM-IM. Thus, successfully materials 65 wt% ratio. concept described study will be single natural substrate, is not initially position.

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ژورنال

عنوان ژورنال: Journal of Oleo Science

سال: 2021

ISSN: ['1345-8957', '1347-3352']

DOI: https://doi.org/10.5650/jos.ess20101